Mechanisms behind variation in the Clostridium difficile 16S–23S rRNA intergenic spacer region
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چکیده
منابع مشابه
Mechanisms behind variation in the Clostridium difficile 16S–23S rRNA intergenic spacer region
Clostridium difficile infection is an increasing problem in hospitals worldwide, mainly due to the recent emergence of a hypervirulent C. difficile strain. C. difficile PCR ribotyping, based on size variation of the 16S-23S rRNA intergenic spacer region (16S-23S ISR), is widely used in Europe for molecular epidemiological investigation. The mechanism underlying the 16S-23S ISR size variations i...
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PCR-ribotyping, a typing method based on size variation in 16S-23S rRNA intergenic spacer region (ISR), has been used widely for molecular epidemiological investigations of C. difficile infections. In the present study, we describe the sequence diversity of ISRs from 43 C. difficile strains, representing different PCR-ribotypes and suggest homologous recombination as a possible mechanism drivin...
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Clostridium difficile is a major spore-forming environmental pathogen that causes serious health problems in patients undergoing antibiotic therapy. Consequently, reliable and sensitive methods for typing individual strains are required for epidemiological and environmental studies. Ribotyping is generally considered the best method, but it fails to account for sequence diversity which might ex...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 2010
ISSN: 0022-2615,1473-5644
DOI: 10.1099/jmm.0.020792-0